Thermo HisPur? Ni-NTA Spin Columns, 0.2 mL 8224
Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.
Features of HisPur Ni-NTA Resin:
? High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
? Versatile—purify proteins using native or denaturing conditions
? Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
? Cost-effective—reuse the same batch of resin at least five times
? Easy to use—pre-formulated buffers available for kit formats
? Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates
The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
| Format: | Spin Column, Microcentrifuge |
|---|---|
| Column Type: | Affinity, Agarose Resin |
| Product Size: | 25 columns |
| Immobilized Phase: | Metal Chelate, Divalent Nickel |
Store at 4°C
